Wittscaping Landscaping owns 13 bee hives behind its building on Third and Monroe Street.
The bees are used to make honey for the owners other business, Sweet Success.
Last month, complaints were made about the beehives to the town council, but they wanted to know more about the safety issues.
While some neighbors have signed the petition, the owner of the business next door has had no problems with the bees.
The Newburgh Town Council will meet tonight to discuss the issue further.